The clip reveals the moment the owner left her phone propped up by a plate to record the mysterious activity.
It shows the arm of the stuffed toy slowly moving below its side.
The woman who recorded the footage claimed everyone in her shop who has touched the doll has fallen sick afterwards.
“I clicked play and this is what happened”
She said: “We received a house clearance and in the boxes were an array of oddities and amazing pieces from around the world.
“One bag had a bunch of old toys as I was checking for good quality toys to sell I came across an old musical raggedy ann doll which straight away reminded me of the real doll based on Annabelle the film.
“I placed her on the floor with her arms on her knees and went down to tell my volunteers what I had found.
“When I came back her arms were at her sides, I thought it was a bit spooky so decided to place her arms back on her knees and take a photo so if she moved I could compare to photo.
“The next day I decided to have my phone on a plate stand to hold it secure and film the doll on a shelf away from any possible causes of movement.
“Every time I left the stock room I would put it on record, and at end of the day when I clicked play and this is what happened.”
The clip was uploaded to YouTube account killerscott666, where it has racked up hundreds of views today.
And viewers were left terrified.
One comment read: “I’m pretty sure that’s breathing I can hear just before phone falls over, creepy.”
Another added: “Glad I got rid of all my dolls.
And a third claimed: “When the camera fell it scared me more than the doll moving.”