Monster hunters Roger Patterson and Rob Gimlin believed they captured the furry giant in northern California, USA, way back in 1967.
The footage they caught has caused fierce debate ever since, but the grainy nature of it has left it nearly impossible to fully analyse its authenticity.
Now, a tech wizard has remastered the famous clip using high definition technology which may finally answer much-needed questions.
The video shows the creature walking through the forest in new clarity, revealing muscles flexing on “Bigfoot’s” leg and thigh.
Viewers claim such a detail wouldn’t be seen if it were just a man wearing a Hollywood-style monkey suit.
The creature’s thumb appears to be in a position that suggests it is not human, as it is located further up its hand.
Bigfoot Al, who edited the clip, also noted on his YouTube channel how there was a “very weird knee movement” that “no human” would do.
At one point, the black figure also appears to stumble – a detail that was missed in the original grainy footage.
Bigfoot experts have been quick to voice their thoughts on the image.
Karl Shuker, a British zoologist, cryptozoologist and author, told the Fortean Times Appreciation page on Facebook: “At the time when this famous film was shot, Walt Disney Studio was at the forefront of special effects in movies and I recall reading that a Disney spokesman claimed that even they did not have the technology back then to create anything as lifelike as the alleged bigfoot in the film.
“Having said that, we can never know with absolute certainty the truth of what it depicts, however much the film is viewed, discussed and analysed.”
Most viewers of the YouTube clip were convinced it was real, with one saying: “There’s no way that’s a dude in a suit. It’s too much detail. Bigfoot has to be real.”
Another added: “Dude just watched your footage – mind officially blown.”
And a third commented: “The only footage that is not a hoax. This is proof and the only evidence of Bigfoot’s existence.”
But one wasn’t convinced, saying: “It still looks like a big man in a bad suit to me.”
The original footage was the subject of a 1998 BBC investigation, in which Chris Packham eventually came to the conclusion that it was a hoax.
This new video, though, will only serve to set the conspiracy flame alight once more.